Biología de la capacitación espermática: un proceso in vivo
Autores
Jorge Alberto Salazar-Cartín
Resumen
La capacitación espermática es un proceso biológico que ocurre de forma natural entre el epidídimo del varón hasta el óvulo de la mujer. A lo largo de este recorrido los espermatozoides son objeto de grandes modificaciones. Estas modificaciones son necesarias para que el espermatozoide adquiera la capacidad de desencadenar una reacción acrosomal y proseguir con la fecundación del óvulo. En la presente revisión se acompaña a los espermatozoides en este recorrido y se revisan los distintos aportes que realizan los órganos sexuales tanto masculinos como femeninos sobre ellos, así como la interacción entre los fluidos de la pareja que se complementan en este íntimo proceso.
Palabras clave
Biología capacitación espermática, reacción acrosomal,fertilidad, transporte espermático, semen, fecundación in vitroAbstract
Sperm capacitation is a biological process that occurs naturally in the journey from the male epididymis until it reaches a woman’s egg. Throughout this journey the sperm suffers abundant modifications which are necessary in order to acquire the ability to trigger an acrosome reaction and to continue with the ovule’s fertilization. In the present work, we deeply reviewed the sperm’s capacitation journey, the different contributions made by the male or female sexual organs, and any cooperative activity of each partner’s fluids that might complement each other in this intimate process.
Key words
Biology sperm capacitation, acrosomal reaction, in vitro fertilization, fertility, sperm transport, semenTexto completo
Referencias
1) Bavister B. Early history of in vitro fertilization. Reproduction. 2002; 124(2) 181-196 https://www.ncbi.nlm.nih.gov/pubmed/12141931
2) Gervasi M, Visconti P. Chang's meaning of capacitation: A molecular perspective. Mol Reprod Dev. 2016; 83(10):860-874 https://doi.org/10.1002/mrd.22663
3) De Jonge, C. Biological basis for human capacitation. Hum Reprod, 2005; 3:205-214 https://doi.org/10.1093/humupd/dmi010
4) De Jonge, C. Biological basis for human capacitation - Revised. Human Reproduction Update. 2017; 23(3):289–299 https://doi.org/10.1093/humupd/dmw048
5) Arenas E, Cambron A, Ambriz D. Bases fisiológicas de la capacitación y de la reacción acrosomal del espermatozoide. ContactoS. 2010; 78: 5–11. https://www.researchgate.net/profile/Ahiezer_Rodriguez Tobon/publication/272887428_Bases_fisiologicas_de_la_capacitacion_y_de_la_reaccion_acrosomal_del_espermatozoide/links/54f265fb0cf24eb879483561/Bases-fisiologicas-de-lacapacitacion-y-de-la-reaccion-acrosomal-del-espermatozoide.pdf
6) Cerezo, G. y Lopez, L. (2014) Análisis Macroscópico del Semen, En:Cerezo, G. et al, Manual para el Análisis Básico de Semen: Una Guía Práctica, 1ra Edición, Editorial Prado, 15 – 20.
7) Olivera, M, Ruiz T, Tarazona A et al. El espermatozoide, desde la eyaculacion hasta la fertilizacion. Rev Col Cienc Pec. 2006; 19:426-436. http://www.scielo.org.co/pdf/rccp/v19n4/v19n4a08.pdf
8) Björndahl L, Mortimer D, Barratt C et al. A Practical Guide to Basic Laboratoy Andrology, 1ra edición, Cambridge University Press; 2010; 167 – 188.
9) Travis A, Kopf G. The role of cholesterol efflux in regulating the fertilization potential of mammalian spermatozoa. J Clin Invest. 2002;110(6):731-6 https://doi.org/10.1172/JCI16392
10) Velazquez, G. Fisiologia de la reproducción humana. Rev Mex Med Rep. 2009; 1:115-130. http://www.medigraphic.com/pdfs/reproduccion/mr-2009/mr094b.pdf
11) Del Rio M, Godoy A, Toro A et al. La reacción acrosomal del espermatozoide: avances recientes. Rev Int Androl. 2007; 5:368-373. https://dialnet.unirioja.es/servlet/articulo?codigo=5783913
12) Arienti G, Carlini E, Saccardi C et al. Role of human prostasomes in the activation of spermatozoa. J Cell Mol Med. 2004; 8:77-84. https://www.ncbi.nlm.nih.gov/pubmed/15090262
13) Palmerini C, Saccardi C, Carlini E et al. Fusion of prostasomes to human spermatozoa stimulates the acrosome reaction. Fertil Steril. 2003; 80:1181-1184. https://www.ncbi.nlm.nih.gov/pubmed/14607571
14) Kerns K, Zigo M, Drobnis E et al. Zinc ion flux during mammalian sperm capacitation. Nature Communications. 2018: 9:2061 https://doi.org/10.1038/s41467-018-04523-y
15) Fraser L, Adeoya-Osiguwa S, Baxendale R et al. First messenger regulation of mammalian sperm function via adenylyl cyclase/cAMP. Journ Reprod Dev. 2005; 51:37-46. https://www.ncbi.nlm.nih.gov/pubmed/15750295
16) Tardif S, Madamidola O, Brown S et al. Clinically relevant enhancement of human sperm motility using compounds with reported phosphodiesterase inhibitor activity. Hum Reprod. 2014; 29(10): 2123-2135. https://doi.org/10.1093/humrep/deu196
17) Kotarska K, Lenartowicz M. Sperm migration and selection in the reproductive tract of female mice is mostly affected by male genotype. Folia Biol (Krakow). 2011; 59(1-2):71-5. https://www.ncbi.nlm.nih.gov/pubmed/21614971
18) Nakano Y, De Barros R, Esteves S. Insights into the role of cervical mucus and vaginal pH in unexplained infertility. MedicalExpress. 2015;2(2): M150207. https://doi.org/10.5935/MedicalExpress.2015.02.07
19) Feki N, Thérond P, Couturier M et al. Human sperm lipid content is modified after migration into cervical mucus, Mol Hum Reprod. 2004; 10:137-142. https://www.ncbi.nlm.nih.gov/pubmed/14742699
20) Ford, W. Regulation of sperm function by reactive oxigen species. Hum Reprod Update. 2004; 10:387-399. https://doi.org/10.1093/humupd/dmh034
21) Kunz G, Beil D, Huppert P et al. Oxytocin - a stimulator of directed sperm transport in humans. Reprod Biomed Online. 2007 Jan;14(1):32-9. https://doi.org/10.1016/S1472-6483(10)60761-4
22) López-Úbeda R, Matás C. An Approach to the Factors Related to Sperm Capacitation Process. Andrology. 2015; 4:128. https://doi.org/10.4172/2167-0250.1000128
23) Gadella B. Sperm membrane physiology and relevance for fertilization. Animal Reproduction Science. 2008; 107: 229–236 https://doi.org/10.1016/j.anireprosci.2008.05.006
24) Lu Y, Huo R, Yuan Y et al. Human testicular protein NYD-SP16 is involved in sperm capacitation and the acrosome reaction. Fertil Steril. 2006; 86(4 Suppl):1228-34 https://doi.org/10.1016/j.fertnstert.2006.05.025
25) Sen S, Chowdhury G, Chowdhury M. Sialic acid binding protein of human endometrium: its regulation by steroids. Mol Cell Biochem. 2001; 221:17-23. https://www.ncbi.nlm.nih.gov/pubmed/11506181
26) Croxatto, H. Physiology of gamete and embryo transport through the fallopian tube. Reprod Biomed Online. 2002; 4:160-169.
27) Yousef M, Marey M, Hambruch N et al. Sperm Binding to Oviduct Epithelial Cells Enhances TGFB1 and IL10 Expressions in Epithelial Cells as Well as Neutrophils In Vitro: Prostaglandin E2 As a Main Regulator of Anti Inflammatory Response in the Bovine Oviduct. PLoS One. 2016; 11(9):e0162309 https://doi.org/10.1371/journal.pone.0162309
28) Zumoffen C, Caille A, Munuce M et al. Proteins from human oviductal tissue-conditioned medium modulate sperm capacitation. Human Reproduction, 2010; 25(6):1504–1512 https://doi.org/10.1093/humrep/deq063
29) Vigil P, Orellana R, Cortés M. Modulation of spermatozoon acrosome reaction. Biol. Res. 2011; 44(2): 151-159 http://dx.doi.org/10.4067/S0716-97602011000200007
30) Kong N, Xu X, Zhang Y et al. Natriuretic peptide type C induces sperm attraction for fertilization in mouse. Scientific Reports. 2017; 7:39711 https://doi.org/10.1038/srep39711
31) Sun, F. et al. Human sperm chemotaxis: both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants. Hum Reprod. 2005; 20(3):761-767
32) Oren-Benroya, R. et al. The sperm chemoattractant secreted from human cumulus cells is progesterone, Hum Reprod. 2008; 23(10):2339-2345
33) Bergqvist A, Ballester J, Johannisson, A et al. In vitro capacitation of bull spermatozoa by oviductal fluid and its components. 2006; 14(3):259-273 https://doi.org/10.1017/S0967199406003777
34) Suarez, S. Interactions of spermatozoa with the female reproductive tract: inspiration forassisted reproduction. Reproduction, Fertility and Development. 2016; 19(1):103-110 https://doi.org/10.1071/RD06101
35) Rath D, Schuberth H, Coy P et al. Sperm Interactions from Insemination to Fertilization.Reprod Dom Anim. 2008; 43(5):2–11 https://doi.org/10.1111/j.1439-0531.2008.01250.x